Mycobacterial Resistance

#topNew Vaccines against TB:
Micobacterial Genetics and Pathogenicity Studies

 
  • Aguilo N, Uranga S, Marinova D, Monzon M, Badiola J, Martin C. MTBVAC vaccine is safe, immunogenic and confers protective efficacy against Mycobacterium tuberculosis in newborn mice. Tuberculosis (Edinb). 2016 Jan;96:71-4. doi: 10.1016/j.tube.2015.10.010. Epub 2015 Nov 30. PubMed PMID: 26786657. [link]

  • Spertini F, Audran R, Chakour R, Karoui O, Steiner-Monard V, Thierry AC, Mayor CE, Rettby N, Jaton K, Vallotton L, Lazor-Blanchet C, Doce J, Puentes E, Marinova D, Aguilo N, Martin C. Safety of human immunisation with a live-attenuated Mycobacterium tuberculosis vaccine: a randomised, double-blind, controlled phase I trial. Lancet Respir Med. 2015 Dec;3(12):953-62. doi: 10.1016/S2213-2600(15)00435-X. Epub 2015 Nov 17. PubMed PMID: 26598141.[link]

  • Aguilo N, Alvarez-Arguedas S, Uranga S, Marinova D, Monzón M, Badiola J, Martin C. Pulmonary but not subcutaneous vaccination confers protection to TB susceptible mice by an IL17-dependent mechanism. J Infect Dis. 2015 Oct 22. pii: jiv503. [Epub ahead of print] PubMed PMID: 26494773.[link]

  • Broset E, Martín C, Gonzalo-Asensio J. Evolutionary Landscape of the Mycobacterium tuberculosis Complex from the Viewpoint of PhoPR: Implications for Virulence Regulation and Application to Vaccine Development. MBio. 2015 Oct 20;6(5). pii: e01289-15. doi: 10.1128/mBio.01289-15. Review. PubMed PMID: 26489860; PubMed Central PMCID: PMC4620462. [link]

  • Gonzalo Asensio J, Aguilo N, Martin C. Nuevas Vacunas contra la Tuberculosis. Investigación y Ciencia. [link]

  • Aguilo N, Toledo AM, Lopez-Roman EM, Perez-Herran E, Gormley E, Rullas-Trincado J, Angulo-Barturen I, Martin C. Pulmonary BCG vaccination confers dose-dependent superior protection than subcutaneous vaccination. Clin Vaccine Immunol. 2014 Feb 5. [Epub ahead of print] PubMed PMID: 24501340. [Link]

  • Arbues A, Aguilo JI, Gonzalo-Asensio J, Marinova D, Uranga S, Puentes E, Fernandez C, Parra A, Cardona PJ, Vilaplana C, Ausina V, Williams A, Clark S, Malaga W, Guilhot C, Gicquel B, Martin C. Construction, characterization and preclinical evaluation of MTBVAC,the first live-attenuated M. tuberculosis-based vaccine to enterclinical trials Vaccine 31 (2013) 4867– 4873. 2013 Aug 16. doi:pii: S0264-410X(13)01008-6. 10.1016/j.vaccine.2013.07.051. [Link] [PPT presentation]

  • Marinova D, Gonzalo-Asensio J, Aguilo N, Martin C. Recent developments in tuberculosis vaccines. Expert Rev Vaccines. 2013 Dec;12(12):1431-48. doi: 10.1586/14760584.2013.856765. PubMed PMID: 24195481[Link]

  • Aguilo J, Alonso H, Uranga S, Marinova D, Arbués A, de Martino A, Anel A, Monzon M, Badiola J, Pardo J, Brosch R, Martin C. ESX-1-induced apoptosis is involved in cell-to-cell spread of Mycobacterium tuberculosis. Cell Microbiol. 2013 Jul 15. doi: 10.1111/cmi.12169. [Epub ahead of print] PubMed PMID: 23848406. [Link]

  • Aguiló N, Marinova D, Martín C, Pardo J. ESX-1-induced apoptosis during mycobacterial infection: to be or not to be, that is the question. Front Cell Infect Microbiol. 2013 Dec 4;3:88. doi: 10.3389/fcimb.2013.00088. eCollection 2013. PubMed PMID: 24364000; PubMed Central PMCID: PMC3850411.[Link]

  • Aporta A, Arbues A, Aguilo JI, Monzon M, Badiola JJ, de Martino A, Ferrer N, Marinova D, Anel A, Martin C, Pardo J. Attenuated Mycobacterium tuberculosis SO2 vaccine candidate is unable to induce cell death. PLoS One. 2012;7(9):e45213. doi: 10.1371/journal.pone.0045213. Epub 2012 Sep 19. PubMed PMID: 23028853; PubMed Central PMCID: PMC3446966.[Link]

  • Jonathan K. Nambiar, Rachel Pinto, Juan I. Aguilo, Kiyoshi Takatsu, Carlos Martin, Warwick J. Britton and James A. Triccas. "Protective immunity afforded by attenuated, PhoPdeficient Mycobacterium tuberculosis is associated with sustained generation of CD41 T-cell memory" 2012 Eur. J. Immunol, 42: 385–392 [Link]

  • Iglesias MJ, Martín C. "Vacunacion frente a Tuberculosis" 2011 An Pediatr Contin. 2011;9(3):186-90 [Link]

  • Joaquín Sanz, Jorge Navarro, Ainhoa Arbués, Carlos Martín, Pedro C. Marijuán, Yamir Moreno. "The Transcriptional Regulatory Network of Mycobacterium tuberculosis " 2011 PLOS ONE Volume: 6 Issue 7 Article Numer: e22178 [Link]

  • Brodin P, Poquet Y, Levillain F, Peguillet I, Larrouy-Maumus G, Gilleron M, Ewann F, Christophe T , Fenistein D, Jang JC , Jang MS, Park SJ, Rauzier J, Carralot JP 1, Shrimpton R, Genovesio A, Gonzalo-Asensio JA ,Puzo G, Martin C, Brosch R, Stewart GR, Gicquel B, Neyrolles O. "High Content Phenotypic Cell-Based Visual Screen Identifies Mycobacterium tuberculosis Acyltrehalose-Containing Glycolipids Involved in Phagosome Remodeling" 2010 PLOS PATHOGENS    Volume: 6    Issue: 9  Article Number: e1001100 [Link]

  • Carlos Martín Montañés, Brigitte Gicquel. "New tuberculosis vaccines" 2010 Enfermedades Infecciosas y Microbiología Clínica. 29(Supl 3):56-61. [Link]

  • Ferrer NL, Gomez AB, Neyrolles O, Gicquel B, Martin C Interactions of Attenuated Mycobacterium tuberculosis phoP Mutant with Human Macrophages. 2010 PLOS ONE    Volume: 5    Issue: 9  Article Number: e12978    Published: SEP 24 [Link]

  • P.J. Cardona, J. Gonzalo Asensio, A. Arbués, I. Otal, C. Lafoz, O. Gil, N. Caceres,V. Ausina, B. Gicquel, C. Martin "Extended safety studies of the attenuated live tuberculosis vaccine SO2 based on phoP mutant" Vaccine 27 (2009) 2499–2505 [Link]

  • Verreck FAW, Vervenne RAW, Kondova I, van Kralingen KW, Remarque EJ, et al. (2009) MVA.85A Boosting of BCG and an Attenuated, phoP Deficient M. tuberculosis Vaccine Both Show Protective Efficacy Against Tuberculosis in Rhesus Macaques. PLoS ONE 4(4): e5264. doi:10.1371/journal.pone.0005264 [Link]

  • Gonzalo-Asensio J, Mostowy S, Harders-Westerveen J, Huygen K, Hernández-Pando R, Thole J, Behr M, Gicquel B, Martín C. PhoP: a missing piece in the intricate puzzle of Mycobacterium tuberculosis virulence. PLoS One. 2008;3(10):e3496. doi: 10.1371/journal.pone.0003496. Epub 2008 Oct 23. PubMed PMID: 18946503; PubMed Central PMCID: PMC2566814. [Link]

  • Gonzalo-Asensio J, Soto CY, Arbués A, Sancho J, Menéndez MD, García MJ, Gicquel B, Martín C. Mycobacterium tuberculosis phoPR operon is positively autoregulated in the virulent strain H37Rv. J Bacteriol. 2008 Aug 29. [Link]

  • Asensio, J.A., Arbues, A., Perez, E., Gicquel, B., and Martin, C. (2008) Live tuberculosis vaccines based on phoP mutants: a step towards clinical trials. Expert Opin Biol Ther 8: 201-211.[Link]

  • Frigui W, Bottai D, Majlessi L, Monot M, Josselin E, et al. (2008) Control of M. tuberculosis ESAT-6 Secretion and Specific T Cell Recognition by PhoP. PLoS Pathog 4(2): e33 doi:10.1371/journal.ppat.0040033[Link]

  • Chesne-Seck, M.L., Barilone, N., Boudou, F., Gonzalo Asensio, J., Kolattukudy, P.E., Martin, C., Cole, S.T., Gicquel, B., Gopaul, D.N., and Jackson, M. (2008) A point mutation in the two-component regulator PhoP-PhoR accounts for the absence of polyketide-derived acyltrehaloses but not that of phthiocerol dimycocerosates in Mycobacterium tuberculosis H37Ra. J Bacteriol 190: 1329-1334.[Link]

  • Aguilar D, Infante E, Martin C, Gormley E, Gicquel B, Hernandez Pando R. "Immunological responses and protective immunity against tuberculosis conferred by vaccination of Balb/C mice with the attenuated Mycobacterium tuberculosis (phoP) SO2 strain." Clin Exp Immunol. 2007 Feb;147(2):330-338.[Link]

  • Pando RH, Aguilar LD, Infante E, et al. "The use of mutant mycobacteria as new vaccines to prevent tuberculosis" TUBERCULOSIS 86 (3-4): 203-210 May-Jul 2006 [Link]

  • Martin C. Tuberculosis vaccines: past, present and future. Curr Opin Pulm Med. 2006 May;12(3):186-91.[Link]

  • Martin C, Williams A, Hernandez-Pando R, et al. "The live Mycobacterium tuberculosis phoP mutant strain is more attenuated than BCG and confers protective immunity against tuberculosis in mice and guinea pigs". Vaccine 24 (17): 3408-3419 Apr 24 2006 [Link]

  • Jesús Gonzalo Asensio, Catarina Maia, Nadia L. Ferrer, Nathalie Barilone, Françoise Laval, Carlos Y. Soto, Nathalie Winter, Mamadou Daffé, Brigitte Gicquel, Carlos Martín, Mary Jackson. "The Virulence-associeted Two-component PhoP-PhoR System Controls the Biosynthesis of Polyketide-derived Lipids in Mycobacterium tuberculosis ". Journal of Bological Chemistry (2006) 281.3: 1313:1316 [Link]

  • P.J. Cardona, C.Y. Soto, C. Martín, B. Giquel, G. Agustí, E. Guirado, T. Sirakova, P. Kolattukudy, E. Julián, M. Luquin. "Neutral-red reaction is related to virulence and cell wall methyl-branched lipids in Mycobacterium tuberculosis ". Microbes and Infection (2005)[Link]

  • Martin, C. "The Dream of a Vaccine Against Tuberculosis. New Vaccines: Improving or Replacing BCG?". European Respiratory Journal(2005):26.1-6. [Link]

  • Williams A, G.J.H., Simon O Clark, Karen E Gooch, Kim A Hatch, Graham A Hall, Kris Huygen, Tom HM Ottenhoff, Kees LMC Franken, Peter Andersen, T. Mark Doherty, Stefan H.E. Kaufmann, Leander Grode, Peter Seiler, Carlos Martin, Brigitte Gicquel, Stewart T. Cole, Priscille Brodin, Alexander S. Pym, Wilfried Dalemans, Joe Cohen, Yves Lobet, Nilu Goonetilleke, Helen McShane, Adrian Hill, Tanya Parish, Debbie Smith, Neil Stoker, Douglas B Lowrie, Gunilla Källenius, Stefan Svenson, Andrzej Pawlowski, Philip D Marsh. "Evaluation of vaccines in the EU TB Vaccine Cluster using a guinea pig aerosol infection model of tuberculosis". Tuberculosis (Edinb) (2005):85(1-2), 29-38 [Link]

  • Andreu, N., Soto, C.Y., Roca, I., Martin, C., and Gibert, I. "Mycobacterium smegmatis displays the Mycobacterium tuberculosis virulence-related neutral red character when expressing the Rv0577 gene". FEMS Microbiol Lett(2004):231.283-289. Microbiol..42.1.212–219.2004. [Link]

  • Soto CY, Menéndez MC, Pérez E, Samper S, Gómez AB, García MJ, Martín C. "IS6110 Mediates Increased Transcription of the phoP Virulence Gene in a Multidrug-Resistant Clinical Isolate Responsible for Tuberculosis Outbreaks". J. Clin. Microbiol..42.1.212–219.2004. [Link]

  • Ludwiczak P, Gilleron M, Bordat Y, Martin C, Gicquel B, Puzo G. “Mycobacterium tuberculosis phoP mutant: lipoarabinomannan molecular structure.” Microbiology (2002).48:3029-3037. [Abstract]

  • Ainsa JA, Martin C, Gicquel B. "Molecular approaches to tuberculosis". Mol Microbiol. 2001 Oct;42(2):561-70. No abstract available.[Link].

  • Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, Niederweis M. Energy transfer between fluorescent proteins using a co-expression system in Mycobacterium smegmatis. Gene. 2001 Oct 31;278(1-2):115-24. [Abstract].

  • Pérez, E., S. Samper, Y. Bordas, C. Guilhot, B. Gicquel and C. Martin, " An essential role for phoP Mycobacterium tuberculosis virulence"(2001) Mol. Microbiol. :41:179187.. [Link].

  • Gavigan, J.A., Aínsa, J.A. Perez, E. Otal I. and Martín C. "Isolation by genetic labeling of a of a new mycobacterial plasmid, pJAZ38 from Mycobacterium fortuitum " (1997). J. Bacteriol. 179: 4115-4122.. [Link].

  • Aínsa, J.A., C. Martín, M. Cabeza, F. de la Cruz and M.V. Mendiola. "Construction of a family of Mycobacterium/Escherichia coli shuttle cloning vectors derived from pAL5000 and pACYC184". (1996). Gene. 176:23-26. [Abstract].

  • C. Guilhot, I. Otal, I. Van Rompaey, C. Martín and B. Gicquel. "Efificient transposition in mycobacteria: Construction of M. smegmatis insertional mutant libraries". (1994). J. Bacteriol. 176: 535-539. [Abstract].

  • C. Guilhot, B. Gicquel and C. Martín "Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000" (1992) FEMS Microbiol. Lett. 98:181-186. [Abstract].

  • C. Martín, J. Timm, J. Rauzier, R. Gómez-Lus, J. Davies and B. Gicquel. "Transposition of an antibiotic resistance element in mycobacteria".(1990). Nature. 345:739-743. [Abstract].


#topMolecular Epidemiology:

  • Millán-Lou MI, Ollé-Goig JE, Tortola MT, Martin C, Samper S. Mycobacterial diversity causing multi- and extensively drug-resistant tuberculosis in Djibouti, Horn of Africa. Int J Tuberc Lung Dis. 2016 Feb;20(2):150-3. doi: 10.5588/ijtld.15.0268. PubMed PMID: 26792464.[Link]

  • Millán-Lou MI, Otal I, Monforte ML, Vitoria MA, Revillo MJ, Martín C, Samper S. In Vivo IS6110 Profile Changes in a Mycobacterium tuberculosis Strain as Determined by Tracking over 14 Years. J Clin Microbiol. 2015 Jul;53(7):2359-61. doi: 10.1128/JCM.00607-15. Epub 2015 May 6. PubMed PMID: 25948604; PubMed Central PMCID: PMC4473185.[Link]

  • Molina-Moya B, Lacoma A, Prat C, Diaz J, Dudnyk A, Haba L, Maldonado J, Samper S, Ruiz-Manzano J, Ausina V, Dominguez J. AID TB resistance line probe assay for rapid detection of resistant Mycobacterium tuberculosis in clinical samples. J Infect. 2014 Oct 12. pii: S0163-4453(14)00305-3. doi: 10.1016/j.jinf.2014.09.010. [Epub ahead of print] PubMed PMID: 25305498[Link]

  • Merker M, Blin C, Mona S, Duforet-Frebourg N, Lecher S, Willery E, Blum M, Rüsch-Gerdes S, Mokrousov I, Aleksic E, Allix-Béguec C, Antierens A, Augustynowicz-Kopec E, Ballif M, Barletta F, Beck HP, Barry CE 3rd, Bonnet M, Borroni E, Campos-Herrero I, Cirillo D, Cox H, Crowe S, Crudu V, Diel R, Drobniewski F, Fauville-Dufaux M, Gagneux S, Ghebremichael S, Hanekom M, Hoffner S, Jiao WW, Kalon S, Kohl TA, Kontsevaya I, Lillebæk T, Maeda S, Nikolayevskyy V, Rasmussen M, Rastogi N, Samper S, Sanchez-Padilla E, Savic B, Shamputa IC, Shen A, Sng LH, Stakenas P, Toit K, Varaine F, Vukovic D, Wahl C, Warren R, Supply P, Niemann S, Wirth T. Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage. Nat Genet. 2015 Jan 19. doi: 10.1038/ng.3195. [Epub ahead of print] PubMed PMID: 25599400.[Link]

  • Cabal A, Strunk M, Domínguez J, Lezcano MA, Vitoria MA, Ferrero M, Martín C, Iglesias MJ, Samper S. Single nucleotide polymorphism (SNP) analysis used for the phylogeny of the Mycobacterium tuberculosis complex based on a pyrosequencing assay. BMC Microbiol. 2014 Feb 3;14:21. doi: 10.1186/1471-2180-14-21. PubMed PMID: 24491224; PubMed Central PMCID: PMC3922597.[Link]

  • Allix-Béguec C, Wahl C, Hanekom M, Nikolayevskyy V, Drobniewski F, Maeda S, Campos-Herrero I, Mokrousov I, Niemann S, Kontsevaya I, Rastogi N, Samper S, Sng LH, Warren RM, Supply P. Proposal of a consensus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci for subtyping of Mycobacterium tuberculosis Beijing isolates. J Clin Microbiol. 2014 Jan;52(1):164-72. doi: 10.1128/JCM.02519-13. Epub 2013 Oct 30. PubMed PMID: 24172154; PubMed Central PMCID: PMC3911419.[Link]

  • Millán-Lou MI, López-Calleja AI, Colmenarejo C, Lezcano MA, Vitoria MA, Del Portillo P, Otal I, Martín C, Samper S. Global Study of IS6110 in a Successful Mycobacterium tuberculosis Strain: Clues for Deciphering Its Behavior and for Its Rapid Detection. J Clin Microbiol. 2013 Nov;51(11):3631-7. doi: 10.1128/JCM.00970-13. Epub 2013 Aug 28. PubMed PMID: 23985924.[Link]

  • Caceres N, Llopis I, Marzo E, Prats C, Vilaplana C, de Viedma DG, Samper S, Lopez D, Cardona PJ. Low dose aerosol fitness at the innate phase of murine infection better predicts virulence amongst clinical strains of Mycobacterium tuberculosis. PLoS One. 2012;7(1):e29010. doi: 10.1371/journal.pone.0029010. Epub 2012 Jan 3. PubMed PMID: 22235258; PubMed Central PMCID: PMC3250398. [Link]

  • Alonso H, Samper S, Martín C, Otal I. Mapping IS6110 in high-copy number Mycobacterium tuberculosis strains shows specific insertion points in the Beijing genotype. BMC Genomics. 2013 Jun 25;14:422. doi: 10.1186/1471-2164-14-422. PubMed PMID: 23800083; PubMed Central PMCID: PMC3701491[Link]

  • Vitoria MA, González-Domínguez M, Salvo S, Crusells MJ, Letona S, Samper S, Sanjoaquín I. Mycobacterium simiae pulmonary infection unmasked during immune reconstitution in an HIV patient. Diagn Microbiol Infect Dis. 2013 Jan;75(1):101-3. doi: 10.1016/j.diagmicrobio.2012.09.004. Epub 2012 Oct 4. PubMed PMID: 23040511[Link]

  • Henar Alonso, Juan Ignacio Aguilo, Sofía Samper, Jose Antonio Caminero, María Isolina Campos-Herrero, Brigitte Gicquel , Roland Brosch , Carlos Martín, Isabel Otal. Deciphering the role of IS6110 in a highly transmissible Mycobacterium tuberculosis Beijing strain, GC1237. 2011 Tuberculosis (2011), doi:10.1016/j.tube.2010.12.007 [Link]
  • Cameo MI, Lezcano MA, Samper S, et al. Detection of multidrug-resistant Mycobacterium tuberculosis in a pediatric patient with choroiditis. Enfermedades infecciosas y microbiologia clinica. 2010 Volume: 28   Issue: 8   Pages: 560-562 [Link]
     

  • Alonso M, Rodriguez NA, Garzelli C, et al. Characterization of Mycobacterium tuberculosis Beijing isolates from the Mediterranean area.2010 BMC MICROBIOLOGY Volume: 10 Article Number: 151[Link]

  • Lopez-Alvarez R, Badillo-Lopez C, Cerna-Cortes JF, et al. First insights into the genetic diversity of Mycobacterium tuberculosis isolates from HIV-infected Mexican patients and mutations causing multidrug resistance. 2010 BMC MICROBIOLOGY   Volume: 10 Article Number: 82 [Link]

  • Rodriguez E, Sanchez LP, Perez S, et al. Human tuberculosis due to Mycobacterium bovis and M. caprae in Spain, 2004-2007. 2009. INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE   Volume: 13   Issue: 12   Pages: 1536-1541[Link]

  • Gavin P, Iglesias MJ, Jimenez MS, et al. Multidrug-Resistant Mycobacterium tuberculosis Strain from Equatorial Guinea Detected in Spain. 2009. EMERGING INFECTIOUS DISEASES   Volume: 15   Issue: 11   Pages: 1858-1860 [Link]

  • Ana Isabel López-Calleja, Patricia Gavín, Ma Antonia Lezcano, Ma Asunción Vitoria, Ma José Iglesias, Joaquín Guimbao, Ma Ángeles Lázaro, Nalin Rastogi, Ma José Revillo, Carlos Martín and Sofia Samper BMC Pulmonary Medicine 2009. Unsuspected and extensive transmission of a drug-susceptible Mycobacterium tuberculosis strain, 9:3 [Link]

  • I Otal , A B. Gomez, K. Kremer, P. de Haas, M J.García, C. Martín and D. van Soolingen.Mapping of IS6110 insertion sites in clinical Mycobacterium bovis isolates in relation to adaptation from the animal to human hosts.Veterinary Microbiology.
    Vol: 129, 333 - 341 (2008) Veterinary Microbiology[Link]

  • Aristimuno, L., Espana, M., Guilarte, A., Ramirez, C., Rojas, J., Gavin, P., Lopez-Calleja, A.I., Lezcano, M.A., Revillo, M.J., Cebollada, A., Martin, C., and Samper, S. (2007) Multidrug-resistant Mycobacterium tuberculosis Beijing/W genotype in Venezuela. J Med Microbiol 56: 1707-1708.[Link]

  • Cacho, J.C., Meixeira, A.P., Cano, I., Soria, T., Martos, A.R., Concheiro, M.S., Samper, S., Gavin, P., and Martin, C. (2007) Recurrent tuberculosis from 1992 to 2004 in the metropolitan area of Madrid, Spain. Eur Respir J.[Link]

  • Lopez-Calleja, A.I., Lezcano, M.A., Vitoria, M.A., Iglesias, M.J., Cebollada, A., Lafoz, C., Gavin, P., Aristimuno, L., Revillo, M.J., Martin, C., and Samper, S. (2007) Genotyping of Mycobacterium tuberculosis over two periods: a changing scenario for tuberculosis transmission. Int J Tuberc Lung Dis 11: 1080-1086.[Link]

  • Otal Isabel, Ana B. Gomez, Kristin Kremer, Petra de Haas, María J. García, Carlos Martín Dick van Soolingen (2007) Mapping of IS6110 insertion sites in Mycobacterium bovis isolates in relation to adaptation from the animal to human host Veterinary Microbiology Veterinary Microbiology 129 (2008) 333–341 [Link]

  • Murcia, M.I., Garcia, M.J., Otal, I., Gomez, A.B., and Menendez, M.C. (2007) Molecular features of Mycobacterium avium human isolates carrying a single copy of IS1245 and IS1311 per genome. FEMS Microbiol Lett 272: 229-237.[Link]

  • Samper, S., and Martin, C. (2007) Spread of extensively drug-resistant tuberculosis. Emerg Infect Dis 13: 647-648.[Link]

  • Vazquez-Gallardo, R., Anibarro, L., Fernandez-Villar, A., Diaz-Cabanela, D., Cruz-Ferro, E., Perez del Molino, M.L., Tunez, V., Samper, S., and Iglesias, M.J. (2007) Multidrug-resistant tuberculosis in a low-incidence region shows a high rate of transmission. Int J Tuberc Lung Dis 11: 429-435. [Link]

  • Liselotte Aristimuño, Raimond Armengol, Alberto Cebollada, Mercedes España, Alexis Guilarte, Carmen Lafoz, María A Lezcano, María J Revillo, Carlos Martín, Carmen Ramírez, Nalin Rastogi, Janet Rojas, Albina Vázques de Salas, Christophe Sola, Sofía Samper "Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela" [Link]

  • J. Cacho Calvo, J. Astray Mochales, A. Pérez Meixeira, A. Ramos Martos, M. Hernando García, M. Sanchez Concheiro, J.R. Domínguez Pérez, A.B. Gómez, S. Samper, C. Martín ”Ten-year population-based molecular epidemiological study of tuberculosis transmission in the metropolitan area of Madrid, Spain”. International Journal Tuberculosis Lung Diseases (2005) :9.11:1236-1241 [Link]

  • S. Samper, M.J. Iglesias, M.J. Rabanaque, L.I. Gómez, M.C. Lafoz, M.S. Jímenez, A. Ortega, M.A. Lezcano, D. Van Soolingen, C. Martín and The Spanish Working Group on MDR-TB: ”Systematic Molecular Characterization of Multidrug-Resistant Mycobacterium tuberculosis Complex Isolates from Spain”. Journal of Clinical Microbiology (2005) :43.3.1220-1227 [Link]

  • Gortazar, C., Vicente, J., Samper, S., Garrido, J.M., Fernandez-De-Mera, I.G., Gavin, P., Juste, R.A., Martin, C., Acevedo, P., De La Puente, M., and Hofle, U. : ”Molecular characterization of Mycobacterium tuberculosis complex isolates from wild ungulates in south-central Spain”. Veterinary Research(2005):35.43-45 [Link]

  • M.C. Lafoz, A.B. Gómez, A. Cebollada, M.J. Iglesias, C. Martín, S. Samper: "Técnicas Moleculares empleadas en el estudio de la tuberculosis". Revista A.E.T.E.L (2004) [Abstract]

  • A. Ramos, A. Noblejas, T. Martín, A. Varela, R. Daza and S. Samper ”Prolonged Survival of an HIV-Infected Patient with Multidrug-Resistant Mycobacterium bovis Infection Treated with Surgical Resection”. Clinical Infectious Diseases 2004; 39:e53–5 [Link]

  • Rad M. E., Bifani, P., C. Martín, K. Kremer, S. Samper, J. Rauzier, B. Kreiswirth, J. Blázquez, M. Jouan, D. Van Soolingen, and B.. Gicquel.:”. Mutations in putative mutator genes of Mycobacterium tuberculosis strains of the W-Beijing family”. Emerging Infectious Diseases (2003) :9 838 :845 [Link]

  • Pena, M.J. , J.A. Caminero, M.I. Campos-Herrero, J.C. Rodríguez, I. García, P. Cabrera, M.J. Torres,B. Lafarga, F Rodríguez Casrtro S. Samper, F. Cañas, D.A. Enarson and C. Martín.”Epidemiology of tuberculosis on Gran Canaria: a 4 year population study using traditional and molecular approaches”.(2003):618 -22 [Abstract]

  • Brosch R, Gordon SV, Marmiesse M, Brodin P, Buchrieser C, Eiglmeier K, Garnier T, Gutierrez C, Hewinson G, Kremer K, Parsons LM, Pym AS, Samper S, van Soolingen D, Cole ST.
    "A new evolutionary scenario for the Mycobacterium tuberculosis complex".
    Proc Natl Acad Sci U S A. 2002 99:3684-9 [Link]

  • García M, Rodrígez JC, Navarro JF, S. Samper, C Martín, Royo G. “Molecular Epidemiology of tuberculosis in Elche, Spain. . Med Microbiol (2002)-77.[Link]

  • Caminero JA, Pena MJ, Campos-Herrero MI, Rodriguez JC, Garcia I, Cabrera P, Lafoz C, Samper S, Takiff H, Afonso O, Pavon JM, Torres MJ, Soolingen DV, Enarson DA, Martin C. Epidemiological Evidence of the Spread of a Mycobacterium tuberculosis Strain of the Beijing Genotype on Gran Canaria Island. Am J Respir Crit Care Med 2001 Oct 1;164(7):1165-1170.. [Link]. [Editorial]

  • Caminero, J.A., Pena, M J, I. Campos-Herrero, , J.C. Rodríguez, , O. Afonso, , C. Martin, J.M. Pavón, M.J. Torres, , M. Burgos, , P.Cabrera, , P. M. Small, , D.A. Enarson." Exogenous Reinfection with Tuberculosis on a European Island with a Moderate Incidence of Disease" (2001). American Journal Respiratorie Critical Care Medicine. 163:717:- 720.. [Link] [Editorial]

  • Rivero A, Marquez M, Santos J, Pinedo A, Sanchez MA, Esteve A, Samper S, Martin C. "High rate of tuberculosis reinfection during a nosocomial outbreak of multidrug-resistant tuberculosis caused by Mycobacterium bovis strain B." (2001). Clin. Inf. Dis. 32:159-161.. [Link].

  • Kremer K, D. van Soolingen, R, Frothingam, W, Haas, P. Hermans, C. Martín, , P. Palittapongarpin, B. Plikaytis, L. Riley, M. Yakrus, J. Musser, and J. Van Embden "Comparison of methods based on different molecular epidemiological markers for typing of Mycobacterium tuberculosis complex strains: interlaboratory study of discriminatory power and reproducibility". (1999). J. Clin. Microbiol. 37: 2607-2618.. [Link].

  • Codina, G., R. Vidal, N. Martín Casabona, M. Miravitlles, C. Martín " Multidrug-resistant tuberculosis caused by "W"-related strains in three immunocompetent foreing-born patients". (1999) Int. J. Tuberc. Lung Dis. 3(1):82. [Link]

  • Samper, S., Iglesias, M.J., Rabanaque, R., Lezcano, M.A., Vitoria, A., Rubio, M.C., Gómez, L., Otal, I., Gómez-Lus, R. and C. Martín. "The molecular epidemiology of tuberculosis in Zaragoza (Spain): a restrospective epidemiological study in 1993".(1998). Int. J. Tuberc. Lung Dis. 2(4):281-287. [Abstract].

  • Samper S., C. Martín, A. Pinedo, A. Rivero, J. Blázquez, F. Baquero, D. van Soolingen and J. van Embden. "Transmission between HIV-infected patients of multidrug-resistant tuberculosis caused by Mycobacterium bovis". (1997). AIDS. 11: 1237-1242. [Abstract].
  • Gutierrez , M. S. Samper, M. S. Jiménez, J.D. van Embden, J. F. García-Marín and C. Martín. "Identificacion by spoligotyping of the caprine genotype of Mycobacterium bovis in humam tuberculosis" (1997) J. Clin. Microbiol. 35: 3328-3330.. [Link].

  • M. Gutiérrez, S. Samper, J. A. Gavigan, J.F. García Marín and C. Martín "Differenciation by molecular typing of Mycobacterium bovis strains causing tuberculosis in calttle and goats" (1995). J. Clin. Microbiol. 33: 2953-2956.. [Link].

  • J. D.A. van Embden, M. D. Cave, J. T. Crawford, J. W. Dale, K. D. Eisenach, B. Gicquel, P. Hermans, C. Martín, R. McAdam, T. Shinnick and P. Small. "Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: Recommendations for a standardized methodology" (1993). J. Clin. Microbiol. 31:406-409. [Abstract].

  • Otal,I, C. Martín, V. Vincent-Lévy-Frebault, D. Thierry, and B. Gicquel."Restriction fragment length polymorphism (RFLP) using IS6110 as an epidemiological marker in tuberculosis". (1991) J. Clin. Microbiol. 29:1252-1254. [Abstract].

#topMycobacterial Resistance

  • Bailo R, Bhatt A, Aínsa JA. Lipid transport in Mycobacterium tuberculosis and its implications in virulence and drug development. Biochem Pharmacol. 2015 Aug 1;96(3):159-67. doi: 10.1016/j.bcp.2015.05.001. Epub 2015 May 16. Review. PubMed PMID: 25986884. [Link]

  • Rodrigues L, Viveiros M, Aínsa JA. Measuring efflux and permeability in mycobacteria. Methods Mol Biol. 2015;1285:227-39. doi: 10.1007/978-1-4939-2450-9_13. PubMed PMID: 25779319. [Link]

  • Lee RE, Hurdle JG, Liu J, Bruhn DF, Matt T, Scherman MS, Vaddady PK, Zheng Z, Qi J, Akbergenov R, Das S, Madhura DB, Rathi C, Trivedi A, Villellas C, Lee RB, Rakesh, Waidyarachchi SL, Sun D, McNeil MR, Ainsa JA, Boshoff HI, Gonzalez-Juarrero M, Meibohm B, Böttger EC, Lenaerts AJ. Spectinamides: a new class of semisynthetic antituberculosis agents that overcome native drug efflux. Nat Med. 2014 Feb;20(2):152-8. doi: 10.1038/nm.3458. Epub 2014 Jan 26. PubMed PMID: 24464186; PubMed Central PMCID: PMC3972818. [Link]

  • Luo X, Pires D, Aínsa JA, Gracia B, Duarte N, Mulhovo S, Anes E, Ferreira MJ. Zanthoxylum capense constituents with antimycobacterial activity against Mycobacterium tuberculosis in vitro and ex vivo within human macrophages. J Ethnopharmacol. 2013 Mar 7;146(1):417-22. doi: 10.1016/j.jep.2013.01.013. Epub 2013 Jan 18. PubMed PMID: 23337743.[Link]

  • Villellas C, Aristimuño L, Vitoria MA, Prat C, Blanco S, García de Viedma D, Domínguez J, Samper S, Aínsa JA. Analysis of Mutations in Streptomycin-Resistant Strains Reveals a Simple and Reliable Genetic Marker for Identification of the Mycobacterium tuberculosis Beijing Genotype. J Clin Microbiol. 2013 Jul;51(7):2124-30. doi: 10.1128/JCM.01944-12. Epub 2013 Apr 24. PubMed PMID: 23616454; PubMed Central PMCID: PMC3697671.[Link]

  • Luo X, Pires D, Aínsa JA, Gracia B, Duarte N, Mulhovo S, Anes E, Ferreira MJ. Zanthoxylum capense constituents with antimycobacterial activity against Mycobacterium tuberculosis in vitro and ex vivo within human macrophages. J Ethnopharmacol. 2013 Mar 7;146(1):417-22. doi: 10.1016/j.jep.2013.01.013. Epub 2013 Jan 18. PubMed PMID: 23337743.[Link]

  • Rodrigues L, Villellas C, Bailo R, Viveiros M, Aínsa JA. Role of the Mmr efflux pump in drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2013 Feb;57(2):751-7. doi: 10.1128/AAC.01482-12. Epub 2012 Nov 19. PubMed PMID: 23165464; PubMed Central PMCID: PMC3553716.[Link]

  • Familiar O, Munier-Lehmann H, Ainsa JA, et al. Design, synthesis and inhibitory activity against Mycobacterium tuberculosis thymidine monophosphate kinase of acyclic nucleoside analogues with a distal imidazoquinolinone.2010. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY   Volume: 45   Issue: 12   Pages: 5910-5918[Link]

  • Ramon-Garcia S, Martin C, Thompson CJ, et al. Role of the Mycobacterium tuberculosis P55 Efflux Pump in Intrinsic Drug Resistance, Oxidative Stress Responses, and Growth ANTIMICROBIAL AGENTS AND CHEMOTHERAPY   2009. Volume: 53 Issue: 9   Pages: 3675-3682[Link]

  • Familiar O, Munier-Lehmann H, Ainsa JA, et al. FURTHER INSIGHTS IN THE INHIBITORY ACTIVITY OF ACYCLIC THYMINE NUCLEOSIDES AGAINST THYMIDINE MONOPHOSPHATE KINASE OF M. TUBERCULOSIS. 2009 DRUGS OF THE FUTURE   Volume: 34   Pages: 138-138   Supplement: Suppl. A  

  • Virginie Mick, María José Rebollo, Ainhoa Lucía, Maríaxz cJesús García, Carlos Martín and José Antonio Aínsa. Transcriptional analysis of and resistance level conferred by the aminoglycoside acetyltransferase gene aac(20)-Id from Mycobacterium smegmatis Journal of Antimicrobial Chemotherapy (2008) 61, 39–45 [Link]

  • Santiago Ramón-García, Carlos Martín, Edda De Rossi and José A. Aínsa. Contribution of the Rv2333c efflux pump (the Stp protein) from Mycobacterium tuberculosis to intrinsic antibiotic resistance in Mycobacterium bovis BCG. Journal of Antimicrobial Chemotherapy (2007) 59, 544–547[Link]

  • Santiago Ramón-García, Isabel Otal, Carlos Martín, Rafael Gomez-Lus, and José A. Aínsa “Novel Streptomycin Resistance Gene from Mycobacterium fortuitum”. Nov. 2006 Antimicrobial Agents and Chemotherapy, p. 3920–3922. [Link].

  • Santiago Ramón-García, Carlos Martín, José A. Ainsa and Edda De Rossi “Characterization of tetracycline resistance mediated by the efflux pum Tap from Mycobacterium fortuitum”. (2006) Journal of Antimicrobial Chemotherapy 57:252-259. [Link].

  • Edda De Rossi, José A. Ainsa and Giovanna Ricardi "Role of mycobacterial efflux transporters in drug resistance: an unresolved question. (2005) FEMS Microbiol Rev 30 (2006) 36-52 [Link]

  • De Rossi E, Arrigo P, Bellinzoni M, Silva PA, Martin C, Aínsa JA, Guglierame P, Riccardi G. “The multidrug transporters belonging to major facilitator Superfamily (MFS) in Mycobacterium tuberculosis”. (2002) Mol Med 11:714-724.[Link].
  • P. E. A. Silva, F. Bigi, M. de la Paz Santangelo, C. Martín, A. Cataldi, and J.A. Aínsa. "Characterization of P55 a multidrug efflux pump from Mycobacterium bovis". Antimicrob. Agents Chemoth. (2001) 45:800-804.. [Link].

  • Aínsa, J.A. M. C. Blokboel, I. Otal, D. Young, K. de Smet and and C. Martín " Molecular cloning and characterisation of Tap, a putative multidrug-resistant efflux pump present in Mycobacterium fortuitum and M. tuberculosis". (1998) J. Bacteriol. 180:5836-5843.. [Link].

  • Aínsa, J.A., E. Pérez, F .X. Berthet, V. Pelicic, B. Gicquel, C. Martín. "Aminoglycoside 2'-N-acetyltransferase (aac(2')- I) genes are universally present in mycobacteria: Characterization of the aac(2')-I genes from Mycobacterium tuberculosis and Mycobacterium smegmatis.". (1997). Mol. Microbiol. 24:231-441. [Abstract].

  • José. A Ainsa, Carlos Martin, Brigite Giquel, Rafael Gómez Lus, "Characterization of the Chromosomal Aminoglycoside 29-N-Acetyltransferase Gene from Mycobacterium fortuitum". (1996). Antimicrobioal Agents and Chemotherapy Oct. 1996, p. 2350–2355 Vol. 40, No. 10 [Link]

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